4.1.99.12 A137T naturally occuring Rib3 mutant which is partially deficient in cytochromes a, a3, and cytochrome b, the respiratory defect elicited by this mutation cannot be explained by a flavin insufficiency based on the following evidence: 1. growth of the aE280/U1 on respiratory substrates is not rescued by exogenous riboflavin, 2. the levels of flavin nucleotides are not significantly different in the mutant and wild type, phenotype, overview 674407 4.1.99.12 C59A site-directed mutagenesis, the mutant shows 70% of wild-type enzyme activity 675342 4.1.99.12 D21E site-directed mutagenesis, inactive mutant 674406 4.1.99.12 D21N site-directed mutagenesis, inactive mutant 674406 4.1.99.12 D30E site-directed mutagenesis, inactive mutant 674406 4.1.99.12 D32A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme -, 728745 4.1.99.12 D92A site-directed mutagenesis, inactive mutant 675342 4.1.99.12 E154D DBPS activity is abolished 716533 4.1.99.12 E163A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme -, 728745 4.1.99.12 E166A site-directed mutagenesis, inactive mutant 675342