3.5.4.42 D188A about 6fold increase in kcat/Km value 739428 3.5.4.42 D188N activity similar to wild-type 739428 3.5.4.42 D303A activity similar to wild-type 739428 3.5.4.42 D303N activity similar to wild-type 739428 3.5.4.42 degradation microorganisms EAA-3 and EAA-4 degrade atrazine substrate at 0.42 mg per l and h in 60 h at 99% efficiency.With glucose supplementation, the overall degradation rates determined for EAA-3 and EAA-4 are 0.42 and 0.40 mg per h and l, respectively. The strains metabolize 2-hydroxyatrazine, desethylatrazine and desisopropylatrazine, but degradation of desisopropyl-2-hydroxyatrazine and desisopropyl desethyl-2-hydroxyatrazine is only observed in presence of glucose -, 739711 3.5.4.42 H219A 4fold increase in kcat value 739428 3.5.4.42 H249A substitution of an active site histidine residue, complete loss of activity 739428 3.5.4.42 K65A more than 15fold increase in kcat/Km value, 30fold increase in kcat for ammelide 739428 3.5.4.42 K65R more than 15fold increase in kcat/Km value, 12fold increase in kcat for ammelide 739428 3.5.4.42 N304A slight increase in kcat for ammelide 739428