3.5.1.97	H278S	site-directed mutagenesis, the mutant shows 80% reduced activity compared to wild-type	-, 757621	
3.5.1.97	L279S	site-directed mutagenesis, the mutant shows 74% reduced activity compared to wild-type	-, 757621	
3.5.1.97	additional information	AHL-mediated virulence factor production in a plant pathogen, Pectobacterium carotovorum, is dramatically attenuated by coculture with MacQ-overexpressing Escherichia coli Origami 2(DE3), whereas Escherichia coli with an empty vector is unable to quench the pathogenicity	755847	
3.5.1.97	additional information	random transposon mutagenesis, screening of the resulting mutants for the loss of AHL acylase activity. The Tn insertion in mutant A6731 is mapped to a gene termed aiiO	-, 714841	
3.5.1.97	additional information	the strain PAO1-DELTApvd is shown to be more virulent than the wild-type strain, confirming the role of PvdQ as a virulence-reducing agent	710886