3.5.1.78 C59A active site mutant 716863 3.5.1.78 C59A inactive 719288 3.5.1.78 C59A site-directed mutagenesis, inactive catalytic site mutant 727627 3.5.1.78 C79A complete loss of amidase activity without affecting synthetase activity 649733 3.5.1.78 additional information construction of a gene disruption mutant that does not produce glutathionylspermidine 727627 3.5.1.78 additional information site-directed mutagenesis indicates that Cys59 is essential for amidase activity 31903