3.4.24.B14 P751A no hydrolysis of succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 667419 3.4.24.B14 P764Y kcat/Km is 2.6fold lower than wild-type enzyme 667419 3.4.24.B14 W774A kcat/Km is 1.1fold lower than wild-type enzyme 667419 3.4.24.B14 W774F kcat/Km is nearly identical to wild-type value 667419