3.4.21.B34	L184C	increase in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin and insulin B chain to more than 150% of the wild-type activity, no activity with casein	652897	
3.4.21.B34	R131E/R132E	slight decrease in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin, strong decrease in activity with insulin B chain, barely detectable activity with casein	652897	
3.4.21.B34	R414C/A643C	decrease in activity with benzoyl-Val-Gly-Arg-7-amido-4-methylcoumarin to 70% of wild-type activity, decrease in activity with insulin B chain as substrate to 50% of wild-type activity, no activity with casein	652897