3.4.21.72	E1605A	inactive	666834	
3.4.21.72	additional information	construction of an isogenic iga mutant DELTAiga by allelic replacement, which shows significantly decreased lethality to pigs. Removal of gene iga contributes to weakening the ability of Streptoccocus suis in breaking through the mucosa barrier of a host and its inability to invade the pigs	-, 718444	
3.4.21.72	additional information	construction of deletion or point mutants lacking the previously defined consensus cleavage site, the mutants are still able for self-cleavage and subsequent extracellular release of mature IgA1 protease	680138	
3.4.21.72	additional information	construction of igaB mutants and igaA/igaB double mutants of strain 11P6H	680133	
3.4.21.72	additional information	creation of four producer strains with effective expression of recombinant proteins (in the form of inclusion bodies) that represent variants of IgA1pr from Neisseria meningitidis serogroup B (strain B44/76) with different primary structures carrying C-terminal histidine label (-LEH6). Protein I is enzymatically active IgA1pr containing M1K2-N963 sequence. Protein IIa (MA28-N963) also possessing specific enzymatic activity is a variant of protein I lacking signal peptide (M1K2-A27). Enzymatically inactive protein IIm is a variant of protein IIa with catalytically active Ser267 residue substituted with an Ala residue. Protein III, a low-molecular-weight fragment of IgA1pr (ME135-H328), is enzymatically inactive and contains a sequence in the IgA1pr region with high density of potential T- and B-cell epitopes	-, 753131	
3.4.21.72	additional information	generation of an isogenic iga knockout mutant strain, analysis of infection efficiency and persistence in infected cells	755202	
3.4.21.72	P1004A/S1005T/P1006A	site-directed mutagenesis, mutation of the cleavage recognition sequence residues	680138	
3.4.21.72	P1006X	site-directed mutagenesis, mutation of a cleavage recognition sequence residue, the mutant is still able for self-cleavage and subsequent extracellular release of mature IgA1 protease	680138	
3.4.21.72	S1005E	site-directed mutagenesis, mutation of a cleavage recognition sequence residue, the mutant is still able for self-cleavage and subsequent extracellular release of mature IgA1 protease	680138	
3.4.21.72	S267A	site-directed mutagenesis, catalytically inactive mutant	-, 753051