3.2.1.B8 additional information construction of a malZ-yvdF fusion enzyme, MalZ has catalytic characteristics similar to those of MAase, hydrolyzing gamma-CD and attacking maltooligosaccharides from the reducing end, but differs in substrate preference, producing glucose from relatively small maltooligosaccharides, G3 and G4. Construction of yvdF, amyX, and yvdF amyX mutant strains, amyX encodes the a debranching enzyme pullulanase. The yvdF mutant exhibits limited degradation of the substrates beta-cyclodextrin and maltoheptaose, oligosaccharides spectrum, overview -, 708926 3.2.1.B8 additional information construction of a malZ-yvdF fusion enzyme, substrate specificity, overview 708926