3.2.1.68	D367A	site-directed mutagenesis of the catalytic nucleophile Asp-367 in ISA1, the mutation abolishes the catalytic activity of the enzyme complex	732750	
3.2.1.68	G608A	the kcat/Km values and the specific activity of the mutant are decreased as compared to the wild type enzyme	-, 751254	
3.2.1.68	G608K	the kcat/Km values and the specific activity of the mutant are decreased as compared to the wild type enzyme	751254	
3.2.1.68	G608V	the kcat/Km values of the mutant are promoted by 49% and the specific activity increases by 33% as compared to the wild type enzyme	-, 751254	
3.2.1.68	additional information	Cassava starch is debranched by treatment with isoamylase and pullulanase and the yield of resistant starch type III, method optimization with respect to starch solids concentration, incubation time, and enzyme concentration using central composite rotatable design	710571	
3.2.1.68	additional information	construction of a single mutant line isa2-2 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity	-, 732655	
3.2.1.68	additional information	construction of single mutant line isa1-1 by T-DNA insertion mutagenesis. The isa1-1 isa2-2 double mutant line is obtained by a cross between the two corresponding single mutants and screening of the progeny by PCR amplification of genomic DNA using a T-DNA end primer and gene-specific primers. The phenotype of the isa1-1 isa2-2 double mutant is complemented but the ZmISA1 activity, Zea mays ISA1 subunit is expressed via transfection with Agrobacterium tumefaciens strain GV3101 in Arabidopsis thaliana lacking endogenous ISA1 or lacking both native ISA1 and ISA2. The maize protein functions in Arabidopsis leaves to support nearly normal starch metabolism in the absence of any native ISA1 or ISA2	-, 732655	
3.2.1.68	additional information	functional complementation of sta8 mutant strains BafV13 and BafO6 with ISA2 or ISA2-HA, overview	-, 732146	
3.2.1.68	additional information	generation of mutant isa2-339	732581	
3.2.1.68	additional information	generation of mutant su1-4582	732581