3.2.1.25	D199A	low activity	680752	
3.2.1.25	D206N	catalytically active mutant enzyme, similar temperature optimum like wild-type enzyme. The high-catalytic turn-over rate by D206N for beta-glucosidase activity makes it a useful enzyme in cellulose degradation at high temperatures	750443	
3.2.1.25	D206N	in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 11fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 60fold higher than that for 4-nitrophenyl beta-D-mannopyranoside	-, 654641	
3.2.1.25	D206Q	mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides	750443	
3.2.1.25	E206G	complete loss of activity	695664	
3.2.1.25	E207S	no hydrolytic activity	750443	
3.2.1.25	E314G	complete loss of activity	695664	
3.2.1.25	E330A	mutant enzyme displays no activity against mannan or 4-nitrophenyl-beta-mannopyranoside	665524	
3.2.1.25	E399S	no hydrolytic activity	750443	
3.2.1.25	E462A	low activity	680752