3.2.1.130	C188A	enzymatic activity can not be fully rescued with a back-engineered Cys188Ala CHO cell endomannosidase	679134	
3.2.1.130	C188S	enzymatic activity can not be fully rescued with a back-engineered Cys188Ser CHO cell endomannosidase	679134	
3.2.1.130	C188W	enzyme activity can be reversed in cells with a back-engineered Cys188Trp CHO cell endomannosidase, in particular N-glycans of alpha1-antitrypsin become fully processed	679134	
3.2.1.130	E154A	the mutant shows near zero activity	-, 732816	
3.2.1.130	E329A	the mutant shows near zero activity	-, 732816	
3.2.1.130	E332A	the mutant shows approximate 50fold decrease in catalytic activity with respect to wild type enzyme	-, 732816	
3.2.1.130	E333Q	inactive	731659	
3.2.1.130	E407D	the mutant has excellent transglycosylation activity and extremely low hydrolytic activity. The minimum motif required for glycosyl acceptor is Man-alpha-(1->2)-Man	750314	
3.2.1.130	additional information	in CHO-K1 cells expressing DELTAsig-rEndo, lacking the whole signal sequence, or DELTATMD-rEndo, lacking the transmembrane domain, endomannosidase is undetectable. DELTACT-rEndo, lacking the cytoplasmic tail, exhibits an ER localization and fails to maintain a type II membrane topology	697210	
3.2.1.130	W188C	molecular defect in CHO cells, is functionally important since it alone results in endoplasmic reticulum mislocalization of endomannosidase and causes greatly reduced in vivo activity	679134