3.1.3.89	D137A	complete loss of activity	722956	
3.1.3.89	D69A	almost complete loss of activity	722956	
3.1.3.89	D77A	complete loss of activity	722956	
3.1.3.89	D90A	the mutant shows no activity	749824	
3.1.3.89	E114A	the mutant shows no activity	749824	
3.1.3.89	E122A	almost complete loss of activity	722956	
3.1.3.89	E145A	the mutant shows almost no activity	749824	
3.1.3.89	E72A	catalytically inactive. Structures complexed with Co2+ and either TMP or dAMP disclose the unusual binding mode of deoxyribonucleotides in the active site	722956	
3.1.3.89	E93A	the mutant shows almost no activity	749824	
3.1.3.89	H111A	the mutant shows slightly reduced activity compared to the wild type enzyme	749824