3.1.11.3 D119A site-directed mutagenesis, inactive mutant 716778 3.1.11.3 E85A site-directed mutagenesis, almost inactive mutant 716778 3.1.11.3 K131A site-directed mutagenesis, inactive mutant 716778 3.1.11.3 K49A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme 716778 3.1.11.3 K76A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme 716778 3.1.11.3 L78A site-directed mutagenesis, the mutant shows a significantly diminished level of activity compared to the wild-type enzyme 716778 3.1.11.3 M53A site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme 716778 3.1.11.3 R137A site-directed mutagenesis, inactive mutant 716778 3.1.11.3 R28A site-directed mutagenesis, mutation of a residue that contact the 5' phosphate of the DNA, inactive mutant 716778 3.1.11.3 R45A site-directed mutagenesis, inactive mutant 716778