2.7.4.7	additional information	complementation of the Escherichia coli DELTA thiD knockout mutant is possible by heterologous expression of gene ThiD2. Strains expressing a standalone ThiD2 protein are 10fold more resistant than wild-type against bacimethrin toxicity. ThiD2 genes confer resistance to bacimethrin	-, 758754	
2.7.4.7	additional information	construction of a thiD knockout mutant strain, complementation of the Escherichia coli DELTA thiD knockout mutant is possible by heterologous expression of gene ThiD2, ThiD2 proteins catalyze phosphorylation of HMP monophosphate, but not of HMP or its toxic analogues and damage products such as bacimethrin and 5-(hydroxymethyl)-2-methylpyrimidin-4-ol. As strictly monofunctional HMP monophosphate kinases (EC 2.7.4.7), ThiD2 proteins eliminate a potentially fatal vulnerability of canonical ThiD, at the cost of the ability to reclaim HMP formed by thiamin turnover	-, 758754