2.7.1.146 A71E production by site-directed mutagenesis 698924 2.7.1.146 A71E the mutant shows slightly decreased kcat compared to the wild type enzyme 698924 2.7.1.146 D17A production by site-directed mutagenesis 698924 2.7.1.146 D17A the mutant shows severely decreased kcat compared to the wild type enzyme -, 698924 2.7.1.146 D433A production by site-directed mutagenesis, loss of PFK activity 698924 2.7.1.146 D433A the mutant shows no acitivity 698924 2.7.1.146 E72A site-directed mutagenesis, the mutant shows reduced glucokinase and phosphofructokinase activities compared to wild-type enzyme 761465 2.7.1.146 K158A production by site-directed mutagenesis 698924 2.7.1.146 K158A the mutant shows slightly decreased kcat compared to the wild type enzyme -, 698924 2.7.1.146 N15A production by site-directed mutagenesis 698924