2.7.1.145 A110D site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme 661583 2.7.1.145 E52D markedly decreased kcat 673584 2.7.1.145 E52H markedly decreased kcat 673584 2.7.1.145 E52Q inactive mutant protein 673584 2.7.1.145 F114A site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme 661629 2.7.1.145 F114Y site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme 661629 2.7.1.145 I102M/N117S/M118V/D208N clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine 636844 2.7.1.145 I29H site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme 661629 2.7.1.145 I29H/F114Y site-directed mutagenesis, active site mutant, inactive mutant 661629 2.7.1.145 I47T/N210D clones harbouring the mutant enzyme are more sensitive to 3'-azido-2',3'-dideoxythymidine and beta-D-arabinofuranosylcytosine 636844