2.5.1.9	A43L	decrease in affinity for substrate 6,7-dimethyl-8-ribityllumazine. A43L replacement causes substantial perturbation of the overall binding site topology	687468	
2.5.1.9	C48A	site-directed mutagenesis, nearly inactive mutant	658346	
2.5.1.9	C48M	site-directed mutagenesis, nearly inactive mutant	658346	
2.5.1.9	C48S	mutation in the activity cavity, causes significant 19F NMR chemical shift modulation of trifluoromethyl derivatives of 6,7-dimethyl-8-ribityllumazine in complex with the protein. Replacement of C48 changes the electron density topology in the N-terminal substrate binding site in the vicinity of C-6 and C-7 atoms of bound ligand	687468	
2.5.1.9	C48S	site-directed mutagenesis, highly decreased activity compared to the wild-type enzyme	658346	
2.5.1.9	D143G	site-directed mutagenesis, soluble protein, too unstable to be purified	637580	
2.5.1.9	D143N	site-directed mutagenesis, soluble protein, too unstable to be purified	637580	
2.5.1.9	D185L	site-directed mutagenesis, low remaining activity	637580	
2.5.1.9	DELTA1-180	variant that lacks the C-terminal extension the coiled-coil and C-terminal peptide (AaRS1-180)	759481	
2.5.1.9	DELTA1-196	variant that lacks the C-terminal extension (1-196). Substantial decrease in association efficiency with lumazine synthase is observed for the truncated variant	759481