2.4.99.12 E276A the mutation causes a decrease in the catalytic activity of the enzyme 723660 2.4.99.12 G30A the mutant is completely inactive 723660 2.4.99.12 H272A/N273A the mutations drastically affect the ability of the enzyme to transfer Kdo 723660 2.4.99.12 R212A the mutation clearly affects but does not completely prevent Kdo transfer activity 723660