2.3.1.268	D145A	mutation in Ser-Asp-His catalytic triad, loss of activity	-, 745690	
2.3.1.268	H295A	mutation in Ser-Asp-His catalytic triad, loss of activity	-, 745690	
2.3.1.268	additional information	construction of an Eat1 knockout mutant and of N-terminally truncated mutants. Modulation of expression of the TCA cycle and electron transport chain genes using a highly multiplexed CRISPRi approach, simultaneous knockdown of ACO2b, SDH2, RIP1, and MSS51 results in a 3.8fold increase in ethyl acetate productivity over the already high natural capacity. Mitochondrial localization is partially lost when the first 14 amino acids are removed with complete cytosolic expression resulting from truncation at the predicted cut site, i.e. mutants Eat1(DELTA1-14) and Eat1(DELTA1-19)	-, 755709	
2.3.1.268	additional information	in Saccharomyces cerevisiae, the genes encoding phosphopantothenate-cysteine ligase, acetyl-CoA synthetase, and alcohol acetyltransferase are overexpressed by inserting the combined strong promoter PGK1p and the terminator PGK1t. The ethyl acetate levels of all engineered strains are enhanced. The final engineered strain CLy12a-ATF1-ACS2-CAB2 has an ethyl acetate yield of max. 610.26 mg/l, and the yield of higher alcohols is significantly decreased. The modification of ethyl acetate metabolic pathway is extremely important for the high level production of ethyl acetate in Saccharomyces cerevisiae	-, 757358	
2.3.1.268	S121A	mutation in Ser-Asp-His catalytic triad, loss of activity	-, 745690