2.1.1.244	D168X	site-directed mutagenesis, the mutation has no effect on methylation	718160	
2.1.1.244	D178A/D181A	site-directed mutagenesis, mutating the residues Asp178 and Asp181 at the lip of the active site to Ala decreases enzyme activity, which is further decreased by reverse-charge mutagenesis to Lys	718160	
2.1.1.244	D180K	site-directed mutagenesis, inactive mutant	736186	
2.1.1.244	D180N	site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type	756877	
2.1.1.244	D180Y	site-directed mutagenesis, inactive mutant	736186	
2.1.1.244	D212N	site-directed mutagenesis, the mutant shows reduced activity compared to wild-type	756877	
2.1.1.244	E213A	site-directed mutagenesis, the mutant shows 15% reduced activity compared to wild-type	756877	
2.1.1.244	H140A	the mutant loses the catalytic activity, but retains binding affinity to the peptide substrate	756463	
2.1.1.244	H140K	site-directed mutagenesis, inactive mutant	736186	
2.1.1.244	additional information	depleting NRMT in 293LT cells, using lentivirus, significantly decreases methylation of endogenous RCC1, while not affecting overall RCC1 level, depleting NRMT in HeLa cells using short interfering RNAs causing the same effects, RCC1 methylation is rescued by expression of murine NRMT-FLAG, which is not targeted by the human shRNA	718160