1.2.1.48	C241S	site-directed mutagenesis, inactive active-site mutant	743336	
1.2.1.48	C244S	the mutant shows about 90% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	D245N	NAD+ cosubstrate binding is occuring but catalytic reduction is diminished	693389	
1.2.1.48	E207Q	site-directed mutagenesis, inactive mutant	743336	
1.2.1.48	E331Q	site-directed mutagenesis, inactive mutant	743336	
1.2.1.48	F456A	the mutant shows about 50% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	F456A/F457A	the mutant shows about 15% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	F457A	the mutant shows about 40% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	G185A	disruption of NAD+ binding/utilisation	693389	
1.2.1.48	G412R	disruption of interaction of side-chains with substrate	693389