1.14.14.17 D335F mutation in FAD II binding site, nonfunctional enzyme 671379 1.14.14.17 D335F random mutagenesis, mutation in the FADII site, inactive mutant 671379 1.14.14.17 D335P mutation in FAD II binding site, nonfunctional enzyme 671379 1.14.14.17 D335P random mutagenesis, mutation in the FADII site, inactive mutant 671379 1.14.14.17 D335W mutation in FAD II binding site, nonfunctional enzyme 671379 1.14.14.17 D335W random mutagenesis, mutation in the FADII site, inactive mutant 671379 1.14.14.17 D407F site-directed mutagenesis, mutant shows 8% of wild-type activity 657764 1.14.14.17 E60A site-directed mutagenesis in the highly conserved motif 1, the E60A variant poorly complements growth of KLN1, and shows reduced activity and about 50fold increased sensitivity to terbinafine and naftifine and 5fold to ketoconazole compared to that in the wild type, and confers temperature-sensitive growth 684491 1.14.14.17 E60Q site-directed mutagenesis in the highly conserved motif 1, the E60A variant poorly complements growth of KLN1, and shows highly reduced activity and about 50fold increased sensitivity to terbinafine and naftifine and 5fold to ketoconazole compared to that in the wild type, and confers temperature-sensitive growth 684491 1.14.14.17 F203A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 684828