1.13.12.9	K478A	catalytic activity 1/200 of the wild-type enzyme	-, 698757	
1.13.12.9	M142A	kcat is 1/10 of the wild-type enzyme, Km is elevated, affinity for oxygen seems decreased	-, 698757	
1.13.12.9	R143A	inactive	-, 727793	
1.13.12.9	R143A	mutant enzyme shows no activity	-, 727793	
1.13.12.9	R143K	mutant enzyme shows 400fold lower activity compared to wild-type. Wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but PAM is not produced by the R143K and Y536F mutants	-, 727793	
1.13.12.9	R143K	the mutant has about 400fold lower activity than the wild type enzyme	-, 727793	
1.13.12.9	Y536A	inactive	727793	
1.13.12.9	Y536A	mutant enzyme shows no activity	727793	
1.13.12.9	Y536F	mutant enzyme shows 17fold lower activity compared to wild-type. Wild-type PAOpt produces higher amounts of phenylacetamide (PAM) than phenylpyruvate (PPV) as expected, but PAM is not produced by the R143K and Y536F mutants	-, 727793	
1.13.12.9	Y536F	the mutant has about 17fold lower activity than the wild type enzyme	727793