1.13.11.55 C101/104A enzyme with decreased specific activity 664994 1.13.11.55 C101/104S enzyme with decreased specific activity 664994 1.13.11.55 C101A about 10% residual activtiy for both oxygenase and reductase activity 765288 1.13.11.55 C101A enzyme with decreased specific activity 664994 1.13.11.55 C101A iron content is 49% of that of the recombinant wild-type enzyme, oxygenase activity is 12.8% of the activity of recombinant wild-type enzyme, reductase activity is 10.3% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C101A/C104A iron content is 124% of that of the recombinant wild-type enzyme, oxygenase activity is 9.7% of the activity of recombinant wild-type enzyme, reductase activity is 15.3% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C101S 1.95 mol iron content per mol subunit, cysteine residue is essential for activity 697350 1.13.11.55 C101S 98.4% loss of activity -, 663712 1.13.11.55 C101S enzyme with decreased specific activity and a proportional decrease in iron content 664994 1.13.11.55 C101S mutant enzyme contains no iron, oxygenase activity is 1.04% of the activity of recombinant wild-type enzyme, reductase activity is 0.5% of the activity of recombinant wild-type enzyme 664994