1.11.1.28 C130S expressed in yield comparable to that of the wild-type protein. Mutation decreases activity to less than 5% of the wild-type enzyme -, 754070 1.11.1.28 C133S expressed in yield comparable to that of the wild-type protein. Mutation completely suppresses the activity -, 754070 1.11.1.28 C154S mutant enzyme loses peroxidase activity. The enzyme shows no activity and no formation of intramolecular disulfide bond upon treatment with hydroperoxides -, 758317 1.11.1.28 C174S mutant enzyme retains about 10% of the wild-type activity -, 754057 1.11.1.28 C176S mutant enzyme retains about 10% of the wild-type activity -, 754057 1.11.1.28 C61S inactive mutant enzyme -, 754057 1.11.1.28 C87S mutant enzyme loses peroxidase activity. The enzyme shows no activity and no formation of intramolecular disulfide bond upon treatment with hydroperoxides -, 758317 1.11.1.28 Cys129 mutant enzyme shows no activity with cumene hydroperoxide -, 754057 1.11.1.28 Cys132 mutant enzyme retains about 5% of the wild-type activity with cumene hydroperoxide -, 754057 1.11.1.28 E118F expressed at 20% of the wild-type expression level. Mutation decreases catalytic activity 754070