1.1.1.269 K160A inactive 711235 1.1.1.269 K214A substantially reduced catalytic efficiencies with the natural substrates in both the oxidative and reductive direction 711235 1.1.1.269 R211A substantially reduced catalytic efficiencies with the natural substrates in both the oxidative and reductive direction 711235 1.1.1.269 S143A residue acts as H-bond donor to substrate and in charge stabilization of the transition state, large increase in Km value 711235 1.1.1.269 Y156A mutation in general acid/base, large increase in Km value 711235 1.1.1.269 Y156F inactive 711235