6.5.1.3	D292E	about 10% of the ligation activity of wild-type enzyme	662241	
6.5.1.3	E296A	about 10% of the ligation activity of wild-type enzyme	662241	
6.5.1.3	Y5A	about 10% of the RNA ligation activity of wild-type enzyme, mutant enzyme displays 39% of the adenylyltransferase activity observed with wild-type enzyme	662241	
6.5.1.3	K54A	about 15% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity	662241	
6.5.1.3	Q106A	about 20% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity	662241	
6.5.1.3	S292N	about 25% of the ligation activity of wild-type enzyme	662241	
6.5.1.3	R155A	about 35% of the ligation activity of wild-type enzyme, mutant enzyme displays 16% of the adenylyltransferase activity observed with wild-type enzyme	662241	
6.5.1.3	D135A	about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 4% of the adenylyltransferase activity observed with wild-type enzyme	662241	
6.5.1.3	S170A	about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 8% of the adenylyltransferase activity observed with wild-type enzyme	662241	
6.5.1.3	E300A	about 50% of the ligation activity of wild-type enzyme	662241