5.1.99.6 D218V natural mutation 746703 5.1.99.6 K192A mutation decreases in vitro epimerase activity by more than 95% but does not affect dehydratase activity, reaction of EC 4.2.1.136. Mutant cells have essentially normal NAD(P)HX dehydratase activity and NAD(P)HX levels. Cells show metabolome changes, particularly in amino acids, which exceed those in cells lacking the entire yjeF gene. Mutant cells also have reduced levels of free (i.e. weakly bound or unbound) pyridoxal 5'-phosphate 747329 5.1.99.6 Lys270del natural mutation 746703 5.1.99.6 Q66Stop natural mutation 746703 5.1.99.6 Tyr59Stop natural mutation 746703