5.1.1.4	C130S	mutation of the catalytic Cys residues abolishes the enzymatic activity but preserves the mitogenic properties of the protein	676832	
5.1.1.4	C300S	mutation of the catalytic Cys residues abolishes the enzymatic activity but preserves the mitogenic properties of the protein	676832	
5.1.1.4	additional information	development of a Trypanosoma vivax specific PCR detection method based on the Trypanosoma vivax proline racemase (TvPRAC) gene. Forward and reverse primers are designed that bind at 764-783 bp and 983-1002 bp of the gene. To assess its specificity, TvPRAC PCR is conducted on DNA extracted from different haemotropic pathogens.Analysis of the analytical sensitivity of the different PCR methods, method evaluation, overview. Usage of Trypanosoma vivax isolates from bovine specimens from Nigeria, Ethiopia and Venezuela	-, 749144	
5.1.1.4	R240W	mutant enzyme shows similar kinetic constants for proline and hydroxyproline as compared to the wild-type enzyme	730678	
5.1.1.4	W241F the mutant enzyme	shows 5.3fold, 430fold, and 5.8fold lower kcat/Km values for trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, and cis-4-hydroxy-D-proline respectively, mainly due to a marked decrease in kcat values, whereas no significant effects are found in the kinetic constants of proline, suggesting that this (hydrophobic and bulky) tryptophan residue plays an importance role in the recognition of hydroxyproline (more hydrophilic and bulky than proline)	-, 730678