4.2.2.19	E243A	site-directed mutagenesis, Ca2+-binding residue, about 3.6fold reduced activity compared to the wild-type enzyme	652553	
4.2.2.19	E243A/E245A	site-directed mutagenesis, Ca2+-binding residue, about 4fold reduced activity compared to the wild-type enzyme	652553	
4.2.2.19	E245A	site-directed mutagenesis, Ca2+-binding residue, about 6fold reduced activity compared to the wild-type enzyme	652553	
4.2.2.19	E333A	PCR site-directed mutagenesis, E333 is a key residue in catalysis, reduced activity	652290	
4.2.2.19	H272A	PCR site-directed mutagenesis, H272 is a key residue in catalysis, reduced activity	652290	
4.2.2.19	K250A	PCR site-directed mutagenesis, inactive mutant	652290	
4.2.2.19	N213Q	site-directed mutagenesis, Ca2+-binding residue, about 6fold reduced activity compared to the wild-type enzyme	652553	
4.2.2.19	R271A	PCR site-directed mutagenesis, R271 is a key residue in catalysis	652290	
4.2.2.19	R271E	site-directed mutagenesis, active site mutant, catalytically inactive	652553	
4.2.2.19	R271K	site-directed mutagenesis, active site mutant, about 10fold reduced activity compared to the wild-type enzyme	652553