3.7.1.4 A126S site-directed mutagenesis 735304 3.7.1.4 A213S the mutant completely loses catalytic activity towards phloretin 750528 3.7.1.4 E164A the mutation completely abolishes the hydrolytic activity towards phloretin -, 750528 3.7.1.4 E273A the mutation completely abolishes the hydrolytic activity towards phloretin -, 750528 3.7.1.4 F218Y site-directed mutagenesis 735304 3.7.1.4 H118A the mutant completely loses catalytic activity towards phloretin -, 750528 3.7.1.4 H133A the kcat value for phloretin is decreased at least by 1 order of magnitude, whereas the corresponding Km value is barely affected compared to the wild type enzyme 750528 3.7.1.4 H160A the mutant completely loses catalytic activity towards phloretin -, 750528 3.7.1.4 H160F the mutant completely loses catalytic activity towards phloretin -, 750528 3.7.1.4 H217A the mutant has a Km value increased by 3fold and a kcat value decreased by 6fold compared to the wild type enzyme 750528