3.6.1.28 W53A 2.5fold increase in catalytic efficiency 733393 3.6.1.28 D37A about 0.5% of wild-type catalytic efficiency 733393 3.6.1.28 K11A about 15% of wild-type catalytic efficiency 733393 3.6.1.28 D147A about 30% of wild-type catalytic efficiency 733393 3.6.1.28 E81A about 30% of wild-type catalytic efficiency 733393 3.6.1.28 Y39A about 30% of wild-type catalytic efficiency 733393 3.6.1.28 Y79A about 5% of wild-type catalytic efficiency 733393 3.6.1.28 K193A about 60% of wild-type catalytic efficiency 733393 3.6.1.28 K85E decreased solubility, reduced catalytic activity 667797 3.6.1.28 K65A more than 1000fold decrease in kcat value 733393