3.6.1.22 additional information construction of AtNUDX19 mutants, Arabidopsis lines SALK_115339 and SALK_135053 containing a T-DNA insert in the AtNUDX19 gene, physiological function analysis. Neither transcript nor protein of AtNUDX19 is detected in the homozygous SALK_135053 line, and very low levels of the AtNUDX19 transcript and protein are observed in the homozygous SALK_115339 line. Thus, the SALK_135053 and SALK_115339 lines are knockout (KO-nudx19) and knockdown (KD-nudx19) mutants of this gene, respectively. The growth of the mutants is similar to that of wild-type plants under normal growth conditions. The ratio of NADPH to total NADP(H) in KD- and KO-nudx19 plants is approximately 2fold higher than that in wild type under both conditions. The lack of AtNUDX19 had no impact on NADH levels. Under normal light intensity, there is also no difference in NAD+ levels between wild-type and mutant plants. Although NAD+ levels are increased in response to high light in wild type, this increase is almost completely inhibits in the nudx19 mutants, which increases the ratio of NADH to total NAD(H). Tolerance of nudx19 mutants to photooxidative stress, phenotypes. The tolerance of nudx19 mutants to photooxidative stress is due to an enhancement in photosynthesis, increase in antioxidant capacity, and/or modulation of the expression of defense genes through changes in the levels and redox states of NAD(P). Effects of AtNUDX19-disruption on nuclear gene expression, overview 758387 3.6.1.22 additional information construction of the Mycobacterium smegmatis msm1946::Tn transposon mutant, single-cell analysis for isoniazid-treated mutant msm1946-NADH pyrophosphatase using microfluidics and automated time-lapse microscopy -, 758393 3.6.1.22 P237Q the mutation prevents dimer formation, and results in a loss of enzymatic activity -, 720498