3.4.23.20 T219A constructed by cassette insertion -, 653829 3.4.23.20 T219A site-directed mutagenesis, comparison of substrate binding to the wild-type enzyme 668813 3.4.23.20 T219G constructed by cassette insertion -, 653829 3.4.23.20 T219G site-directed mutagenesis, comparison of substrate binding to the wild-type enzyme 668813 3.4.23.20 T219S constructed by cassette insertion -, 653829 3.4.23.20 T219S site-directed mutagenesis, comparison of substrate binding to the wild-type enzyme 668813 3.4.23.20 T219V constructed by cassette insertion -, 653829 3.4.23.20 T219V site-directed mutagenesis, comparison of substrate binding to the wild-type enzyme 668813