3.4.21.9	C112S	site-directed mutagenesis, replacement of the free cysteine residue with serine improves the refolding yield of the recombinant enzyme by 50%. The heat stability of this C112S variant was also significantly improved by supercharging	718352	
3.4.21.9	E136Y	the mutant shows clearly reduced activity compared to the wild type enzyme	732855	
3.4.21.9	E136Y/R213L	the mutant shows about wild type activity	732855	
3.4.21.9	E173A	site directed mutagenesis	716743	
3.4.21.9	E173K	site directed mutagenesis	716743	
3.4.21.9	F144A	site directed mutagenesis	716743	
3.4.21.9	F144S	site directed mutagenesis	716743	
3.4.21.9	H24Q	the mutant shows slightly reduced activity compared to the wild type enzyme	732855	
3.4.21.9	K63A	site directed mutagenesis	716743	
3.4.21.9	K63E	site directed mutagenesis	716743