3.3.2.13 A244G site-directed mutagenesis 753274 3.3.2.13 E338A inactive 725723 3.3.2.13 E338Q inactive 725723 3.3.2.13 F226A the mutant shows 2.5% activity compared to the wild type enzyme 725723 3.3.2.13 R228A the mutant shows 224.5% activity compared to the wild type enzyme 725723 3.3.2.13 V209Q site-directed mutagenesis 753274 3.3.2.13 Y215F the mutant shows 12% activity compared to the wild type enzyme 725723