3.2.1.145 E112A site-directed mutagenesis, catalytically inactive mutant, the mutation eliminates the galactan hydrolysis activity of the enzyme while it does not disrupt its ligand binding ability 732396 3.2.1.145 E112Q site-directed mutagenesis, catalytically inactive mutant, the mutation eliminates the galactan hydrolysis activity of the enzyme while it does not disrupt its ligand binding ability 732396 3.2.1.145 additional information construction of deletion mutants NDELTA42, NDELTA42CDELTA257, NDELTA42CDELTA457, and NDELTA42CDELTA76 showing 100%, 80.1%, 122%, and 8.45% activity relative to wild-type enzyme activity -, 731184