3.1.3.56 C939S eliminates isoprenylation of enzyme which reduces activity towards phosphatidylinositol 4,5-bisphosphate 646393 3.1.3.56 C409S predominantly soluble 646395 3.1.3.56 DELTA407-411 predominantly soluble 646395 3.1.3.56 R343A increased Km and dramatic loss of activity 646396 3.1.3.56 R350A increased Km 646396 3.1.3.56 C409S decreased activity 646397 3.1.3.56 DELTA1-119 no significant influence on localization of enzyme 646399 3.1.3.56 DELTA1-311 no significant influence on localization of enzyme 646399 3.1.3.56 DELTA1-433 enzyme mainly in cytoplasm 646399 3.1.3.56 DELTA725-1001 enzyme exclusively cytoplasmic 646399