3.1.21.4	D134N/K190N/E204V	inactive	730491	
3.1.21.4	D140A	no detectable activity in dsDNA nicking, mutant R1 subunit/wild-type R2 subunit	751757	
3.1.21.4	D140A/E167A/K169A	no detectable activity in dsDNA nicking, mutant R1 subunit/wild-type R2 subunit	751757	
3.1.21.4	D144A	no detectable activity in ssDNA nicking, mutant R2 subunit/wild-type R1 subunit	751757	
3.1.21.4	D146A	no detectable activity in dsDNA nicking, mutant R2 subunit/wild-type R1 subunit	751757	
3.1.21.4	D146A/E177A	slight detectable activity in dsDNA nicking, mutant R2 subunit/wild-type R1 subunit	751757	
3.1.21.4	D146A/E177A/K179A	no detectable activity in dsDNA nicking, mutant R2 subunit/wild-type R1 subunit	751757	
3.1.21.4	D148A	only traces of DNA cleavage activity when used in large excess	666483	
3.1.21.4	D148G	binds DNA with 2times reduced affinity compared to wild-type protein	666483	
3.1.21.4	D148G	only traces of DNA cleavage activity when used in large excess	666483