2.8.1.4	C265A	cells expressing the C265A variant show 88% decrease in their 4-thiouridine level. The mutant enzyme contains no detectable amount of persulfide	725494	
2.8.1.4	C268A	cells expressing the C265A variant contain no 4-thiouridine. The mutant enzyme contains no detectable amount of persulfide	725494	
2.8.1.4	C348A	cells expressing the C348A variant show 33% decreases in their 4-thiouridine levels. The mutant enzyme contains no detectable amount of persulfide	725494	
2.8.1.4	C78A	cells expressing the C78A variant show 8% decrease in their 4-thiouridine levels. The C78A variant has similar amounts of persulfide as the wild-type protein	725494	
2.8.1.4	additional information	Ctu1 (cytosolic tRNA thiouridilase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, the complex is essential to maintaining genome stability	694891	
2.8.1.4	additional information	Ctu1 (cytosolic tRNA thiouridylase) associates with the conserved Ctu2 protein and this complex is the wobble uridine-34 thiolase of cytosolic tRNAs in eukaryotes, the complex is essential to maintaining genome stability	694891	
2.8.1.4	additional information	single or double deletion mutants of the fission yeast ctu1 and ctu2 genes results in a thermosensitive lethal phenotype reminiscent of the inactivation of their worm counterparts	694891