2.3.1.158 additional information construction of pdat, diacylglycerol O-acyltransferases (EC 2.3.1.20) dgat1 and dgat2 single mutants, of dgat1/dgat2 double mutant, and of a dgat1/dgat2/pdat triple mutant, which show 70%, 57%, 36%, 18% and 13%, respectively, of wild-type total lipid% per dry cell weight, phenotypes, overview -, 721119 2.3.1.158 additional information construction of transgenic plants overexpressing the endogenous gene in leaves and roots, the transgenic plants show elevated enzyme activity 660241 2.3.1.158 additional information lipid analysis of enzyme knockout mutant by T-DNA insertion in locus At5g13640. Fatty acid content and composition in seeds does not show significant changes in the mutant. PDAT activity encoded by At5g13640 is not a major determinant of triacylglycerol synthesis in Arabidopsis seeds 676641 2.3.1.158 additional information MiPDAT synthesizes TAG based on functional complementary experiments in the mutant yeast strain H1246 and the membrane lipid phosphatidylcholine (PC) is preferentially used as substrates as revealed by in vitro enzyme activity assay -, 758363