2.1.1.B75	D257G	random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group	713037	
2.1.1.B75	additional information	the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview. In order to increase the availability of malonyl-CoA, a critical precursor of 7-O-methyl aromadendrin, genes for the acyl-CoA carboxylase alpha- and beta-subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinica were also introduced	-, 718652