2.1.1.56	C173A/S174A	modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C	677246	
2.1.1.56	C382Y	15-50% of wild-type activity	735335	
2.1.1.56	C382Y	72% of wild-type methylation activity	734586	
2.1.1.56	C414R	complete loss of methylation activity	734586	
2.1.1.56	C414R	mutation abolishes the N7-MTase activity on GTP	735335	
2.1.1.56	C416R	mutation abolishes the N7-MTase activity on GTP	735335	
2.1.1.56	D122A/S123A	site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, only slightly at 37°C	659921	
2.1.1.56	D144A	TbCmt1 mutant shows 5% of wild-type activity	677098	
2.1.1.56	D146A	mutant completely loses the N-7 activity (methylation of N-7 position of guanine) and 2'-O methylation activity. Mutation defective in N-7 methylation is lethal for DNV replication	721100	
2.1.1.56	D1924A	the mutant shows severely reduced activity compared to the wild type enzyme	757853