2.1.1.179 additional information analysis of sequence–function relationships of Sgm MTase by limited proteolysis and site-directed and random mutagenesis 702415 2.1.1.179 additional information construction of a series of in-frame knockout and knock-in mutants of Escherichia coli, corresponding to the genotypes rsmF+, DELTArsmF, rsmF+ rmtC+, and DELTArsmF rmtC+, mutant strain growth kinetics, overview -, 718596 2.1.1.179 H54A inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin 757224 2.1.1.179 H54E inactive. Mutation dos not impact 30S binding affinity. Mutant strain is sensitive to kanamycin and gentamicin 757224 2.1.1.179 E205A mutant retains S-adenosyl-L-methionine binding 704280 2.1.1.179 E267A mutant retains S-adenosyl-L-methionine binding 704280 2.1.1.179 K199A mutant retains S-adenosyl-L-methionine binding 704280 2.1.1.179 R236A mutant retains S-adenosyl-L-methionine binding 704280 2.1.1.179 D156A mutant with drastically increased sensitivity to kanamycin 702415 2.1.1.179 D158A mutant with drastically increased sensitivity to kanamycin 702415