2.1.1.170 additional information a rsmG null mutant is streptomycin-resistant 721042 2.1.1.170 N39A inactive 733472 2.1.1.170 additional information mutations within the gene gidB confer low-level streptomycin resistance. gidB mutations emerge spontaneously at a high frequency of 0.000001 and, once emerged, result in vigorous emergence of high-level streptomycin-resistant mutants at a frequency more than 2000 times greater than that seen in wild-type strains -, 705767 2.1.1.170 C172S/A14C site-directed mutagenesis -, 779252 2.1.1.170 H53A site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme 721042 2.1.1.170 R139K site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme 721042 2.1.1.170 R139A site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and catalytically inactive 721042 2.1.1.170 D56A site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme 721042 2.1.1.170 R197A site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme 721042 2.1.1.170 K165A site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme 721042