1.7.7.1 C514S almost complete loss of activity 394417 1.7.7.1 C518S almost complete loss of activity 394417 1.7.7.1 G212S/L213T/Y214L/S217C/C220I/S221N mutations mimic partially isoform SiRA -, 742810 1.7.7.1 G513A increased Km for nitrite compared to the wild type enzyme 394417 1.7.7.1 G513E loss of most of the activity 394417 1.7.7.1 G513V loss of most of the activity 394417 1.7.7.1 G519A 68% activity of wild type enzyme 394417 1.7.7.1 G519E 8% activity of wild type enzyme 394417 1.7.7.1 G519V 9% activity of wild type enzyme 394417 1.7.7.1 K449Q mutation does not affect enzymatic activity 726450