1.20.1.1 A146S increases the half-life of thermal inactivation at 45°C from around 1 min to 8 min 691424 1.20.1.1 A176R strongly decreased Km for NADP+ 657956 1.20.1.1 A319E increases the half-life of thermal inactivation at 45°C from around 1 min to 2 min 691424 1.20.1.1 A319E/T101A increases the half-life of thermal inactivation at 45°C from around 1 min to 5 min 691424 1.20.1.1 A325V thermostability almost identical to that of the wild-type enzyme 691424 1.20.1.1 C336D increases solubility and activity, thermostability almost identical to that of the wild-type enzyme 691424 1.20.1.1 D13E increases solubility and activity, thermostability almost identical to that of the wild-type enzyme 691424 1.20.1.1 D13E/M26I/E175A/E332N/C336D mutant obtained by directed evolution, round 4 673144 1.20.1.1 D13E/M26I/E175A/T181S/A308T/E332N/C336D mutant obtained by directed evolution, round 6, strong decrease in KM value for NADP compared to wild-type 673144 1.20.1.1 D13E/M26I/E175A/T181S/E332N/C336D mutant obtained by directed evolution, round 5 673144