1.14.15.8 A106T kcat/Km for conversion of 11-deoxycortisol is 3.17fold higher than wild-type value, 1.43fold increase in progesterone conversion 693956 1.14.15.8 A106T/Q189K/T399S/R409L kcat/Km for conversion of 11-deoxycortisol is 4.3fold higher than wild-type value, 1.13fold increase in progesterone conversion 693956 1.14.15.8 A106T/R409L kcat/Km for conversion of 11-deoxycortisol is 3fold higher than wild-type value, 1.43fold increase in progesterone conversion 693956 1.14.15.8 A243S mutant engineered for steroid 9alpha- and 6beta-hydroxylation, 10fold increase in the selectivity towards 6alpha-hydroxylation of progesterone. Mutant produces 3.4% 15beta-hydroxy-progesterone plus 4.6% 11alpha-hydroxy-progesterone plus 2.4% 9alpha-hydroxy-progesterone plus 82.7% 6beta-hydroxy-progesterone 746507 1.14.15.8 A395L Vmax/Km for progesterone is 2fold lower than wild-type value 697323 1.14.15.8 D153V/I214F kcat/Km for conversion of 11-deoxycortisol is 2.64fold higher than wild-type value, progesterone conversion is 93% of wild-type value 693956 1.14.15.8 D217V kcat/Km for conversion of 11-deoxycortisol is 2.68fold higher than wild-type value, 1.18fold increase in progesterone conversion 693956 1.14.15.8 E90V/D185G Vmax/Km for progesterone is 1.3fold higher than wild-type value 697323 1.14.15.8 F165I 1.25fold increase in progesterone conversion 693956 1.14.15.8 F165L/A395E/G397V mutant engineered for steroid 9alpha- and 6beta-hydroxylation, 10fold increase in the selectivity towards progesterone 9lpha-hydroxylation. Mutant produces 11.3% 15beta-hydroxy-progesterone plus 17.2% 11alpha-hydroxy-progesterone plus 59.7% 9alpha-hydroxy-progesterone plus 6.8% 6beta-hydroxy-progesterone 746507