1.14.11.6 D191A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 D215S mutant proteins fail to restore the J-level in the JBP1 null trypanosomes to wild-type levels 689246 1.14.11.6 D216N site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 D393A site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain 700501 1.14.11.6 D416A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect 700084 1.14.11.6 E122A site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 F292A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 745921 1.14.11.6 H189A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 H189A/D191A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 H213S mutant proteins fail to restore the J-level in the JBP1 null trypanosomes to wild-type levels 689246