1.13.11.60 A891G site-directed mutagenesis, the mutant converts linoleic acid to 7,8-DiHODE as major product and 8,11-DiHODE as minor product, which is the similar pattern as the wild-type enzyme -, 741712 1.13.11.60 C1005S the variant exhibits only 8-HPODE formation without 6,8-DiHODE accumulation 764229 1.13.11.60 C1006S the hydroperoxide isomerase activity of 5,8-LDS mutant is abolished 711024 1.13.11.60 C1006S/V328F the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts 711024 1.13.11.60 C1006S/V328L the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts 711024 1.13.11.60 C1006S/V328S the mutant forms after reduction of hydroperoxides to alcohols, (8R)-hydroperoxy-(9Z,12Z)-octadecadienoic acid and (10R)-hydroxy-(8E,12Z)-octadecadienoic acid in the same relative amounts 711024 1.13.11.60 C1038S site-directed mutagenesis, the mutant produces a low amount of 7,8-DiHODE with the accumulation of 8-HPODE 741712 1.13.11.60 D749A the mutant shows less than 10% activity of the wild type enzyme 764229 1.13.11.60 E384A mutant forms only traces of (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate 704475 1.13.11.60 E728A the mutant shows less than 10% activity of the wild type enzyme 764229